The dynamic response of upstream DNA to transcription-generated torsional stress

@article{Kouzine2004TheDR,
  title={The dynamic response of upstream DNA to transcription-generated torsional stress},
  author={Fedor Kouzine and J. Liu and S. Sanford and Hye-Jung Chung and David Levens},
  journal={Nature Structural &Molecular Biology},
  year={2004},
  volume={11},
  pages={1092-1100}
}
  • Fedor Kouzine, J. Liu, +2 authors David Levens
  • Published 2004
  • Biology, Medicine
  • Nature Structural &Molecular Biology
  • The torsional stress caused by counter-rotation of the transcription machinery and template generates supercoils in a closed topological domain, but has been presumed to be too short-lived to be significant in an open domain. This report shows that transcribing RNA polymerases dynamically sustain sufficient torsion to perturb DNA structure even on linear templates. Assays to capture and measure transcriptionally generated torque and to trap short-lived perturbations in DNA structure and… CONTINUE READING
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