The dynamic process of apoptosis analyzed by flow cytometry using Annexin-V/propidium iodide and a modified in situ end labeling technique.

@article{Span2002TheDP,
  title={The dynamic process of apoptosis analyzed by flow cytometry using Annexin-V/propidium iodide and a modified in situ end labeling technique.},
  author={Lambert F. R. Span and Arie H.M. Pennings and Gertie Vierwinden and Jan Bernardus Maria Boezeman and Reinier A. P. Raymakers and Theo de Witte},
  journal={Cytometry},
  year={2002},
  volume={47 1},
  pages={24-31}
}
BACKGROUND To study the apoptotic process in time, we used the following flow cytometric (FCM) techniques: phosphatidylserine (PS) translocation by Annexin-V (AnV), DNA fragmentation by in situ end labeling (ISEL), and propidium iodide (PI) staining. Because PS translocation is assumed to be an early feature of programmed cell death (PCD), we questioned if AnV positivity implies inevitable cell death. METHODS Apoptosis was induced in Jurkat cells by gamma-irradiation, incubation with… CONTINUE READING
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