The duplicated gene copy of the ovine growth hormone gene contains a PvuII polymorphism in the second intron.

@article{Gootwine1993TheDG,
  title={The duplicated gene copy of the ovine growth hormone gene contains a PvuII polymorphism in the second intron.},
  author={E Gootwine and J. A. Sise and J. M. Penty and Grant W. Montgomery},
  journal={Animal genetics},
  year={1993},
  volume={24 4},
  pages={319-21}
}
PvuII restriction fragment length polymorphism (RFLP) was found at the growth hormone locus in sheep carrying the GH2 allele where the gene is duplicated. By restriction analysis and using the polymerase chain reaction we demonstrated that this RFLP is due to a mutation at the PvuII site located in the second intron of the 3' copy of the GH2 allele.