The draft genome and transcriptome of Cannabis sativa

@article{vanBakel2011TheDG,
  title={The draft genome and transcriptome of Cannabis sativa},
  author={Harm van Bakel and Jake M. Stout and Atina G. Cot{\'e} and Carling M Tallon and Andrew G. Sharpe and Timothy R. Hughes and Jonathan E. Page},
  journal={Genome Biology},
  year={2011},
  volume={12},
  pages={R102 - R102}
}
BackgroundCannabis sativa has been cultivated throughout human history as a source of fiber, oil and food, and for its medicinal and intoxicating properties. Selective breeding has produced cannabis plants for specific uses, including high-potency marijuana strains and hemp cultivars for fiber and seed production. The molecular biology underlying cannabinoid biosynthesis and other traits of interest is largely unexplored.ResultsWe sequenced genomic DNA and RNA from the marijuana strain Purple… 
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Phylogenetic analysis suggests a history of duplication and divergence affecting drug content, and the diversity of THCA and CBDA synthase sequences observed in the mapping population, the position of enzyme coding loci on the map, and patterns of expression suggest multiple linked loci.
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A meta-transcriptomic analysis of cannabis was used to identify genes for aromatic prenyltransferases of the UbiA superfamily and chalcone isomerase-like (CHIL) proteins and showed CBGAS activity in both N. benthamiana and S. cerevisiae.
A physical and genetic map of Cannabis sativa identifies extensive rearrangements at the THC/CBD acid synthase loci.
TLDR
A combined physical and genetic map derived from a cross between the drug-type strain Purple Kush and the hemp variety "Finola" reveals that cannabinoid biosynthesis genes are generally unlinked but that aromatic prenyltransferase (AP), which produces the substrate for THCA and CBDA synthases (THCAS and CBDAS), is tightly linked to a known marker for total cannabinoid content.
A New and Improved Genome Sequence of Cannabis sativa
TLDR
The Cannbio-2 genome sequence assembly was found to be the most complete genome sequence available based on nucleotides assembled and BUSCO evaluation in Cannabis sativa with a comprehensive genome annotation.
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