The double-strand-break repair model for recombination

@article{Szostak1983TheDR,
  title={The double-strand-break repair model for recombination},
  author={Jack W. Szostak and Terry L. Orr-Weaver and Rodney Rothstein and Franklin William Stahl},
  journal={Cell},
  year={1983},
  volume={33},
  pages={25-35}
}
DNA double-strand break repair: genetic determinants of flanking crossing-over.
TLDR
It is demonstrated that a double-strand gap can be repaired through gene conversion copying a homologous duplex, as predicted by the double-Strand break repair models, in the RecE pathway of Escherichia coli.
Evidence for conservative (two-progeny) DNA double-strand break repair.
TLDR
The results demonstrate the presence of the conservative (two-progeny) double-strand break repair mechanism and set up experimental conditions that should favor detection of conservative ( two-proGeny) recombination.
Genetic recombination through double-strand break repair: shift from two-progeny mode to one-progeny mode by heterologous inserts.
TLDR
It is demonstrated that reciprocal or conservative recombination is frequent at a double-strand break even when there exists the alternative route of nonreciprocal or nonconservative recombination (two parental DNAs resulting in only one progeny DNA).
DNA strand-exchange patterns associated with double-strand break-induced and spontaneous mitotic crossovers in Saccharomyces cerevisiae
TLDR
HtDNA patterns in spontaneous COs isolated in either a wild-type background or in a background with elevated levels of reactive oxygen species were similar to those associated with the DSB-induced events, suggesting that DSBs are the major instigator of spontaneous mitotic recombination in yeast.
Gene conversion adjacent to regions of double-strand break repair
TLDR
The use of restriction site mutations in the his3 gene is described to detect conversion events flanking but outside of a region of a double-strand break repair, consistent with heteroduplex DNA tracts of at least several hundred base pairs adjacent to regions of double-Strand break Repair.
Roles of double-strand breaks in generalized genetic recombination.
  • F. Stahl
  • Biology
    Progress in nucleic acid research and molecular biology
  • 1986
Recombinational Repair of DNA Damage inEscherichia coli and Bacteriophage λ
  • A. Kuzminov
  • Biology
    Microbiology and Molecular Biology Reviews
  • 1999
TLDR
It is now appreciated that DNA repair and homologous recombination are related through DNA replication, and knowledge about recombinational repair in the broader context of DNA replication will guide future experimentation.
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