The double-strand-break repair model for recombination

  title={The double-strand-break repair model for recombination},
  author={Jack W. Szostak and Terry L. Orr-Weaver and Rodney Rothstein and Franklin William Stahl},

DNA double-strand break repair: genetic determinants of flanking crossing-over.

It is demonstrated that a double-strand gap can be repaired through gene conversion copying a homologous duplex, as predicted by the double-Strand break repair models, in the RecE pathway of Escherichia coli.

DNA End-Processing and Heteroduplex DNA Formation During Recombinational Repair of DNA Double-Strand Breaks

This article will provide an account of what is currently known about homologous recombination and its DNA repair role and suggest that the former appears to be the more critical mechanism in the G1 phase and the latter taking on a prominent role in late S and G2 when a sister chromatid becomes available to direct the repair process.

Evidence for conservative (two-progeny) DNA double-strand break repair.

The results demonstrate the presence of the conservative (two-progeny) double-strand break repair mechanism and set up experimental conditions that should favor detection of conservative ( two-proGeny) recombination.

Repairing a double-strand chromosome break by homologous recombination: revisiting Robin Holliday's model.

The establishment of a modified replication fork during DSB repair links gene conversion to another important repair process, break-induced replication, and the essential ideas he proposed of strand invasion and heteroduplex DNA formation, the formation and resolution of HJs, and mismatch repair remain the basis of thinking.

DNA strand-exchange patterns associated with double-strand break-induced and spontaneous mitotic crossovers in Saccharomyces cerevisiae

HtDNA patterns in spontaneous COs isolated in either a wild-type background or in a background with elevated levels of reactive oxygen species were similar to those associated with the DSB-induced events, suggesting that DSBs are the major instigator of spontaneous mitotic recombination in yeast.

Gene conversion adjacent to regions of double-strand break repair

The use of restriction site mutations in the his3 gene is described to detect conversion events flanking but outside of a region of a double-strand break repair, consistent with heteroduplex DNA tracts of at least several hundred base pairs adjacent to regions of double-Strand break Repair.

Roles of double-strand breaks in generalized genetic recombination.

  • F. Stahl
  • Biology
    Progress in nucleic acid research and molecular biology
  • 1986



Yeast recombination: the association between double-strand gap repair and crossing-over.

  • T. Orr-WeaverJ. Szostak
  • Biology
    Proceedings of the National Academy of Sciences of the United States of America
  • 1983
The outcome of double-strand gap repair in plasmids that are capable of autonomous replication and therefore allow recovery of both crossover and noncrossover products is described.

Yeast transformation: a model system for the study of recombination.

Consideration of models for plasmid integration and gene conversion suggests that RAD52 may be involved in the DNA repair synthesis required for these processes and implications for the isolation of integrative transformants, fine-structure mapping, and the cloning of mutations are discussed.

Mismatch repair in heteroduplex DNA.

  • J. WildenbergM. Meselson
  • Biology, Chemistry
    Proceedings of the National Academy of Sciences of the United States of America
  • 1975
Heteroduplex DNA with base pair mismatches was prepared by annealing mixtures of genetically marked DNA from bacteriophage lambda used to transfect bacteria under conditions minimizing recombination.

A mechanism of DNA transposition.

  • R. HarsheyA. I. Bukhari
  • Biology
    Proceedings of the National Academy of Sciences of the United States of America
  • 1981
A mechanism by which integrative replication ofacteriophage Mu and many other transposable elements undergo transposition by a process that involves replication of the element is described.

A hypothesis for the initiation of genetic recombination in eukaryotes

A hypothesis of initiation is proposed that will accommodate experimental observations, which involves the recognition of initiation sites by an endonuclease capable of migrating along a DNA duplex and that cuts in trans.

Hybrid DNA formation during meiotic recombination.

This behavior suggests that G234 blocks the migration of the symmetric phase of hybrid DNA that diffuses from the high conversion end but does not prevent the formation of asymmetrical hybrid DNA.

Effects of the RAD52 Gene on Recombination in SACCHAROMYCES CEREVISIAE.

The RAD52 gene function is required for spontaneous mitotic recombination, UV- and gamma-ray-induced mitotic Rewind recombination and meiotic recombination in rad52/rad52 diploids.

Evidence for meiotic recombination in Ascobolus involving only one member of a tetrad.

The analysis of intragenic recombination at the his, locus in yeast by FOGEL and HURST (1967) yielded a second observation which was difficult to explain by the hybrid DNA models.