The double pH optimum of 5'-nucleotidase of bull seminal plasma.

@article{Levin1966TheDP,
  title={The double pH optimum of 5'-nucleotidase of bull seminal plasma.},
  author={Stephen M. Levin and O Bodansky},
  journal={The Journal of biological chemistry},
  year={1966},
  volume={241 1},
  pages={51-6}
}
The action of bull seminal plasma .5’-nucleotidase on adenosine 5’-phosphate in the presence of magnesium has one optimum at pH 7.5 to 8.0 and a second at pH 9.1 to 9.3. Only one optimum, at pH 7.5 to 8.0, occurs in the absence of magnesium. The phenomenon of a double pH optimum was independent of the nature of the buffer that was employed. A magnesium… CONTINUE READING