The dnaK protein of Escherichia coli possesses an ATPase and autophosphorylating activity and is essential in an in vitro DNA replication system.

@article{ylicz1983TheDP,
  title={The dnaK protein of Escherichia coli possesses an ATPase and autophosphorylating activity and is essential in an in vitro DNA replication system.},
  author={Maciej Żylicz and Jonathan H LeBowitz and Roger McMacken and Costa P. Georgopoulos},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1983},
  volume={80 21},
  pages={6431-5}
}
The Escherichia coli dnaK gene product, originally defined by mutations that blocked lambda phage DNA replication, is known to be necessary for E. coli viability. We have purified dnaK protein to homogeneity and have demonstrated that it possesses a weak DNA-independent ATPase activity, which results in the production of ADP and Pi. The proof that this… CONTINUE READING