The disulphide bond structure of thyroid-stimulating hormone β-subunit

Abstract

Previously only one of the six disulphide bonds within the βsubunit of bovine thyrotropin (bTSHβ) has been unequivocally assigned. In the present investigation, the fluorescent alkylating reagent 5-N-[(iodoacetamidoethyl)amino]naphthalene-1-sulphonic acid has been employed as part of a double-alkylation strategy to allow the relative reactivities and the… (More)

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