• DOI:10.1042/BJ1100407
• Corpus ID: 6499366

The distribution of the components of mixed-function oxidase between the rough and the smooth endoplasmic reticulum of liver cells.

@article{Holtzman1968TheDO,
  title={The distribution of the components of mixed-function oxidase between the rough and the smooth endoplasmic reticulum of liver cells.},
  author={Jordan L. Holtzman and Theodore E. Gram and Philippe Gigon and James R. Gillette},
  journal={The Biochemical journal},
  year={1968},
  volume={110 3},
  pages={
          407-12
        }
}

• J. Holtzman, T. E. Gram, +1 author J. Gillette
• Published 1 December 1968
• Biology, Computer Science, Chemistry
• The Biochemical journal

Mixed-function oxidase activity, when measured by the N-demethylation of ethylmorphine or the hydroxylation of aniline, is significantly higher in the smooth hepatic endoplasmic reticulum than in the rough. In the rabbit the smooth membrane/rough membrane activity ratios are significantly greater than 1 whether the activities are expressed per g. of liver (ratio 5), per mg. of protein (ratio 3-5), per mug. of phospholipid phosphorus (ratio 2), per unit of cytochrome P-450 (ratio 1.7) or per unit of NADPH-cytochrome c reductase activity (ratio 2). On the other hand, if the activities are normalized to the NADPH-cytochrome P-450 reductase, there is no significant difference between the rough and smooth membranes. These results suggest that, in the rabbit, the rate-limiting step is the reduction of cytochrome P-450. In contrast, in the rat the difference in activities can be explained by differences in the concentration of cytochrome P-450.