The distribution of cells containing estrogen receptor-alpha (ERalpha) and ERbeta messenger ribonucleic acid in the preoptic area and hypothalamus of the sheep: comparison of males and females.

@article{Scott2000TheDO,
  title={The distribution of cells containing estrogen receptor-alpha (ERalpha) and ERbeta messenger ribonucleic acid in the preoptic area and hypothalamus of the sheep: comparison of males and females.},
  author={Christopher J Scott and Alan John Tilbrook and Donna M. Simmons and John A. Rawson and S Chu and Peter James Fuller and Nancy H Ing and Iain James Clarke},
  journal={Endocrinology},
  year={2000},
  volume={141 8},
  pages={
          2951-62
        }
}
We have used in situ hybridization to compare the distributions of estrogen receptor alpha (ERalpha) and ERbeta messenger RNA (mRNA)-containing cells in the preoptic area and hypothalamus of ewes and rams. Perfusion-fixed brain tissue was collected from luteal phase ewes and intact rams (n = 4) during the breeding season. Matched pairs of sections were hybridized with sheep-specific, 35S-labeled riboprobes, and semiquantitative image analysis was performed on emulsion-dipped slides. A number of… CONTINUE READING

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