The distribution of ascorbic acid between various cellular components of blood, in normal individuals, and its relation to the plasma concentration

@article{Evans1982TheDO,
  title={The distribution of ascorbic acid between various cellular components of blood, in normal individuals, and its relation to the plasma concentration},
  author={Roger M. Evans and L Currie and A. E. R. Campbell},
  journal={British Journal of Nutrition},
  year={1982},
  volume={47},
  pages={473 - 482}
}
1. A study was undertaken to investigate the distribution of ascorbic acid between various cellular components of blood, in normal individuals, and its relation to the plasma concentration. Forty-one unsupplemented individuals and sixteen supplemented (2 g/d for 5 d) individuals were studied. 2. Granulocytes, mononuclear leucocytes, platelets and erythrocytes were separated by differential sedimentation and centrifugation. Ascorbic acid contents were measured by the dinitrophenylhydrazine… 
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References

SHOWING 1-10 OF 59 REFERENCES
Relationship Between Leucocyte and Plasma Ascorbic Acid Concentrations
Leucocyte and plasma ascorbic acid values were measured in healthy students, adult factory employees, and old people not receiving supplementary vitamin C and in healthy old people receiving 500 mg
Effect of Platelets on Apparent Leucocyte Ascorbic Acid Content
TLDR
It is suggested that changes in the relative distribution of platelets and leucocytes in the blood will result in an apparent change in the ‘leucocyte’ ascorbic acid content regardless of any actual change inThe ascorBic Acid content of the cells.
Utilization of Ascorbic Acid During Platelet Aggregation 1
  • M. Sahud, P. M. Aggeler
  • Biology, Medicine
    Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine
  • 1970
TLDR
Ascorbic acid is not released into the plasma during platelet aggregation induced by various agents and is not merely present for tissue transport and appears to serve some definite metabolic function in Platelet aggregation.
An improved method for the measurement of leucocyte ascorbic acid concentrations.
  • H. S. Loh, C. W. Wilson
  • Medicine, Biology
    International journal for vitamin and nutrition research. Internationale Zeitschrift fur Vitamin- und Ernahrungsforschung. Journal international de vitaminologie et de nutrition
  • 1971
An improved method for measuring leucocyte ascorbic acid concentrations which employs sodium metrizoate instead of dextraven for separation of red blood cells out of the supernatant fluid is
The effect of leucocytosis on leucocyte ascorbic acid levels.
TLDR
There was no correlation between white-blood counts and polymorph counts and plasma ascorbic-acid concentrations, but it is suggested that this phenomenon represents migration of asCorbate-rich polymorphs to areas of infection and infarction.
Reassessment of changes in leucocyte and serum ascorbic acid after acute myocardial infarction.
TLDR
Observations on normal subjects given infusions of tetracosactrin (Synacthen) show that adrenal stimulation can produce a similar rise in white blood cells and an apparent fall in leucocyte ascorbic acid concentration with the exception that the serum ascorBic acid remains unaltered.
The effect of ascorbic acid supplementation on some parameters of the human immunological defence system.
  • W. Prinz, R. Bortz, B. Bregin, M. Hersch
  • Medicine, Biology
    International journal for vitamin and nutrition research. Internationale Zeitschrift fur Vitamin- und Ernahrungsforschung. Journal international de vitaminologie et de nutrition
  • 1977
TLDR
It was shown that ascorbic acid supplementation caused a statistically significant increase in the serum levels of IgA, IgM and C-3 complement, and the study does not permit of conclusions regarding the mechanisms of action of ascorBic acid.
Platelet ascorbic acid levels in normal subjects and in disease
TLDR
The platelet ascorbic acid concentration was measured in 26 normal subjects and found to be 20 times as high as in plasma, suggesting that a platelet defect may be responsible for the low platelets of patients with uraemia, leukaemia, and megaloblastic anaemia.
Changes in Leucocyte Ascorbic Acid during the Common Cold
TLDR
The effect of such supplements of ascorbic acid in 4 episodes of the common cold in 3 subjects suggests that the L.A.A.'s pattern can be changed by this therapy.
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