The discovery of new intron-containing human tRNA genes using the polymerase chain reaction.

@article{Green1990TheDO,
  title={The discovery of new intron-containing human tRNA genes using the polymerase chain reaction.},
  author={Christopher J M Green and I Sohel and B. S. Vold},
  journal={The Journal of biological chemistry},
  year={1990},
  volume={265 21},
  pages={
          12139-42
        }
}
Introns in transfer RNA genes are rare in vertebrates. Until now, the only intron-containing human tRNA genes were believed to be those coding for tRNA(Tyr). All of these introns are inserted 3' to the anticodon position in these genes. We have designed polymerase chain reaction primers that can amplify all of the tRNA(Tyr) genes for cloning and sequencing by using the conserved portions of the gene coding for the structural part of the tRNA. Our preliminary results have revealed five tRNA(Tyr… CONTINUE READING