The dinucleosome as an initial product of chromatin cleavage by endogenous endonucleases

Abstract

The existence of dinucleosomes, or elementary supranucleosomal structures of chromatin, was discovered by the writers with the use of an endonuclease from B~evibacterium ammoniagenes [i]. The endonuclease produces cleavage of rat liver chromatin DNA by single-stranded breaks in each second linker, and this leads to a decrease in the effectiveness of action of endogenous nuclear nucleases [i, 3]. Meanwhile, other investigators demonstrated the dinucleosomal organization of chromatin from sea urchin sperm [6], rat liver [7], and chick erythrocytes [7, i0]. Unmodified [6~ i0] or ferritin-bound pancreatic DNase I [7] was used as the probe. in all the investigations cited above exogenous endonucleases were used. Endogenous enzymes under these circumstances either were inhibited [7, 8], their effect weakened by the use of short-term hydrolysis by high concentrations of exogenous nuclease [6], or tissues in which endogenous activity was absent were used [4, 7, 8, i0].

DOI: 10.1007/BF00834425

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@article{Basnakyan2004TheDA, title={The dinucleosome as an initial product of chromatin cleavage by endogenous endonucleases}, author={A. G. Basnak'yan and N. V. Bubnov and Igor I. Votrin}, journal={Bulletin of Experimental Biology and Medicine}, year={2004}, volume={101}, pages={498-501} }