• Corpus ID: 215542348

The developmental capacity of nuclei taken from intestinal epithelium cells of feeding tadpoles.

  title={The developmental capacity of nuclei taken from intestinal epithelium cells of feeding tadpoles.},
  author={J. B. Gurdon},
  journal={Journal of embryology and experimental morphology},
  • J. Gurdon
  • Published 1 December 1962
  • Biology
  • Journal of embryology and experimental morphology
An important problem in embryology is whether the differentiation of cells depends upon a stable restriction of the genetic information contained in their nuclei. The technique of nuclear transplantation has shown to what extent the nuclei of differentiating cells can promote the formation of different cell types (e.g. King & Briggs, 1956; Gurdon, 1960c). Yet no experiments have so far been published on the transplantation of nuclei from fully differentiated normal cells. This is partly because… 

Nuclear Transplantation in Amphibia and the Importance of Stable Nuclear Changes in Promoting Cellular Differentiation

  • J. Gurdon
  • Biology
    The Quarterly Review of Biology
  • 1963
This article reviews the question of whether stable changes restricting the developmental capacity of cell neclei are important in promoting the normal differentiation of somatic cells and argues that some results support, and others are consistent with, the following general conclusions.

Nuclear transplantation from intestinal epithelial cells of early and late Xenopus laevis tadpoles.

It was concluded that the developmental potential of the gut nuclei is restricted relative to that of the blastula nuclei, but that these restrictions are reversed in a small proportion of cases.

Formation of the blastocyst: determination of trophoblast and embryonic knot.

  • H. Denker
  • Biology
    Current topics in pathology. Ergebnisse der Pathologie
  • 1976
In mammalian development, the earliest apparent differentiation of cells into two distinct lines manifests itself in the early blastocyst, when trophoblast and embryonic knot (“inner cell mass”)

Genomic potential of differentiated cells analyzed by nuclear transplantation

Results of nuclear transplantation experiments in amphibian oocytes and eggs suggest that the genetic totipotency of at least some differentiated somatic cell types still remains a tenable hypothesis.

Cell specialization in the epithelium of the small intestine of feeding Xenopus laevis tadpoles.

The intestinal epithelium of feeding Xenopus laevis tadpoles was studied using light microscope, electron microscope and autoradiographic techniques and indicates that there are no differences between the cells in their degree of specialization.

Cell proliferation in the intestinal epithelium of Xenopus laevis tadpoles

It is suggested that all specialized cells can divide and that proliferation is not localized in particular regions of the epithelium as it is in other vertebrates.

The developmental potentialities of regeneration blastema cell nuclei as determined by nuclear transplantation.

  • A. Burgess
  • Biology
    Journal of embryology and experimental morphology
  • 1967
There is now abundant evidence that blastema cells arise by the dedifferentiation of some of the stump tissues, in particular the stump muscle and cartilage, and that contributions may also arise from fibroblasts when they are present.

The developmental capacity of nuclei transplanted from keratinized skin cells of adult frogs.

It is concluded that cell specialization does not involve any loss, irreversible inactivation or permanent change in chromosomal genes required for development.



Transplantation of Living Nuclei From Blastula Cells into Enucleated Frogs' Eggs.

  • R. BriggsT. J. King
  • Biology
    Proceedings of the National Academy of Sciences of the United States of America
  • 1952
The role of the nucleus in embryonic differentiation has been the subject of investigations dating back to the beginnings of experimental embryology, and the known cytogenetical mechanisms that could account for nuclear differentiation have been indicated.

Factors responsible for the abnormal development of embryos obtained by nuclear transplantation in Xenopus laevis.

  • J. Gurdon
  • Biology
    Journal of embryology and experimental morphology
  • 1960
The extent to which technical and innate factors are responsible for abnormalities of transplant-embryo development has been analysed and nuclei from early donor stages have been used, since these nuclei are believed to have the innate capacity for entirely normal development.

The Effects of Ultraviolet Irradiation on Uncleaved Eggs of Xenopus Laevis

The effects are described of ultraviolet (u.v.) irradiation upon the eggs of Xenopus laevis to facilitate the analysis of nuclear transplantation experiments in Xenopus, and the increased penetrability of eggs is of technical value for this purpose.

A description of the technique for nuclear transplantation in Xenopus laevis.

Differences between the eggs of Rana and Xenopus have made it necessary to modify the technique before it can be satisfactorily applied to the Eggs of Xenopus, and the extent to which these modifications might affect a direct comparison of the results of transplantation experiments is discussed.

Regeneration of the Lens in the Amphibian Eye

  • R. Reyer
  • Biology
    The Quarterly Review of Biology
  • 1954
This review will concern itself only with the regeneration of the lens of the Amphibia, which is not a simple structure but a very complex, highly differentiated photoreceptor capable of mediating vision of considerable acuity.

Paramutation and Chromosome Organization

It is concluded that possibly paramutation may be a very general phenomenon that operates as an information exchanges during differentiation and development of the organism.

Normal table of Xenopus laevis (Daudin). A systematical and chronological survey of the development from the fertilized egg till the end of metamorphosis.

A Systematical and Chronological Survey of the Development from the Fertilized Egg till the End of Metomorphosis

Paramutation and chromosome organization.

Two processes, mutation and paramutation, are postulated to account for the observed heritable changes in the immediate R region of chromosome 10 in maize. Mutation is sporadic, undirected, and

Serial transplantation of embryonic nuclei.