The development of human sera tests for HDL-bound serum PON1 and its lipolactonase activity.

@article{Gaidukov2007TheDO,
  title={The development of human sera tests for HDL-bound serum PON1 and its lipolactonase activity.},
  author={Leonid Gaidukov and Dan S. Tawfik},
  journal={Journal of lipid research},
  year={2007},
  volume={48 7},
  pages={1637-46}
}
Serum paraoxonase (PON1) is a lipolactonase that associates with HDL-apolipoprotein A-I (HDL-apoA-I) and thereby plays a role in the prevention of atherosclerosis. Current sera tests make use of promiscuous substrates and provide no indications regarding HDL-PON1 complex formation. We developed new enzymatic tests that detect total PON1 levels, irrespective of HDL status and R/Q polymorphism, as well as the degree of catalytic stimulation and increased stability that follow PON1's tight binding… CONTINUE READING