The development of ghost vaccines trials

@article{Batah2020TheDO,
  title={The development of ghost vaccines trials},
  author={Ali M. Batah and Tarek Adnan Ahmad},
  journal={Expert Review of Vaccines},
  year={2020},
  volume={19},
  pages={549 - 562}
}
ABSTRACT Introduction Bacterial ghosts are intact bacterial cell envelopes that are emptied of their content by gentle biological or chemical poring methods. Ghost techniques increase the safety of the killed vaccines, while maintaining their antigenicity due to mild preparation procedures. Moreover, ghost-platforms may express and/or carry several antigens or plasmid-DNA encoding for protein epitopes. Areas covered In this review, the development in ghost-vaccine production over the last 30… 

Bacterial Ghosts-Based Vaccine and Drug Delivery Systems

The preparation methods for BGs, interactions between BGs and the host, and research progress in BG development are reviewed and further highlighted.

Bacteria from Infectious Particles to Cell Based Anticancer Targeted Drug Delivery Systems

The current review explores the impact of BGs on the field of drug delivery and drug targeting, their synthesis, their uniqueness as a delivery system and application principles in cancer are discussed.

A Novel and Efficient High-Yield Method for Preparing Bacterial Ghosts

A novel and commonly used method to produce high-quality BGs on a large scale for the first time is described and it is shown that the expression level of protein EM was significantly higher than that of the non-pLysS plasmid.

Peptidoglycan-Free Bacterial Ghosts Confer Enhanced Protection against Yersinia pestis Infection

To develop a modern plague vaccine, hypo-endotoxic Yersinia pestis bacterial ghosts with combinations of genes encoding the bacteriophage lysis-mediating protein E and/or holin-endolysin systems from λ or L-413C phages resulted in BGs that retained the shape of the original bacterium.

Efficient Robust Yield Method for Preparing Bacterial Ghosts by Escherichia coli Phage ID52 Lysis Protein E

A novel and highly efficient method for BGs preparation depending on recombinant expression of E. coli phage ID52-E under eco-friendly and reasonable price inducer L-arabinose is introduced.

Proteomic and Antibody Profiles Reveal Antigenic Composition and Signatures of Bacterial Ghost Vaccine of Brucella abortus A19

Brucellosis is an important zoonotic disease that causes great economic losses. Vaccine immunisation is the main strategy for the prevention and control of brucellosis. Although live attenuated

Lactobacillus casei Ghosts as a Vehicle for the Delivery of DNA Vaccines Mediate Immune Responses

Results indicate L. casei BGs effectively mediate immune responses and can be used as delivery system for DNA vaccination.

Immunogenicity and protective efficacy of the recombinant Pastureland multocida lipoproteins VacJ and PlpE, and outer membrane protein H from P. multocida A:1 in ducks

It was shown through histopathological examination and tissue bacterial load detection that all vaccines could reduce tissue damage and bacterial colonization to varying and indicated that recombinant PlpE or OmpH fusion proteins formulated with oil adjuvants have the potential to be used as vaccine candidates against duck cholera subunits.

Prodigiosin-Functionalized Probiotic Ghosts as a Bioinspired Combination Against Colorectal Cancer Cells

PG-LAGs provide a novel bacteria-based combination for anticancer biomedicine and are evaluated as a bioinspired formulation against colorectal cancer (CRC) using LAGs functionalized with prodigiosin.

References

SHOWING 1-10 OF 229 REFERENCES

Bacterial ghosts as carrier and targeting systems for mucosal antigen delivery

The extended bacterial ghost system represents a platform technology for specific targeting of DNA-encoded antigens to primary antigen-presenting cells and offers a significant technical advantage, especially when used as combination vaccines.

Antigen discovery and delivery of subunit vaccines by nonliving bacterial ghost vectors

The bacterial ghost (BG) platform system is a novel vaccine delivery system endowed with intrinsic adjuvant properties that enhance the immune response to target antigens, including T-cell activation and mucosal immunity.

Applications of bacterial ghosts in biomedicine.

Bacterial Ghosts represent a promising technology platform for novel vaccines including combination or DNA vaccines, as drug carriers for therapeutic approaches in tumor treatment and as novel probiotics, as carrier of enzymatic activity.

Bacterial ghosts as adjuvant particles

Owing to the particulate nature of BGs and the fact that they contain many well known immune-stimulating compounds, BGs have the potential to enhance immune responses against ghost-delivered target antigens.

Bacterial Ghosts Are an Efficient Delivery System for DNA Vaccines1

Bacterial ghosts not only target the DNA vaccine construct to APC, but also provide a strong danger signal, acting as natural adjuvants, thereby promoting efficient maturation and activation of dendritic cells in the development of more efficient DNA vaccines.

Bacterial ghosts as adjuvants: mechanisms and potential

Understanding of BG interactions with the host immune system, their exploitation as an adjuvant and a delivery system, and address important areas of future research interest are summarized.

Bacterial ghosts: non-living candidate vaccines.

T cell-specific immune response induced by bacterial ghosts.

It is found that antigen-presenting cells have the capacity to stimulate specific T cells after the internalization and processing of Actinobacillus ghosts, as demonstrated by a strong specific T-cell response generated against the ghost antigens.
...