The development of antisera to prostaglandins B2 and F2alpha and their analysis using solid-phase and double antibody radioimmunoassay methods.

@article{Dighe1975TheDO,
  title={The development of antisera to prostaglandins B2 and F2alpha and their analysis using solid-phase and double antibody radioimmunoassay methods.},
  author={K K Dighe and Heather A. Emslie and Lynette K. Henderson and Lester Simon},
  journal={British journal of pharmacology},
  year={1975},
  volume={55 4},
  pages={
          503-14
        }
}
1 A method for the production of highly substituted prostaglandin-bovine serum albumin conjugates has been developed. 2 Antisera to prostaglandins B2 and F2alpha were raised in rabbits immunized with prostaglandin-bovine serum albumin conjugates. 3 The antisera were assessed for specificity and sensitivity by the double antibody radioimmunoassay method and after they were covalently linked to powdered cellulose to form a 'solid-phase' system. 4 Solid phase radioimmunoassays were developed using… CONTINUE READING