The development of a real-time PCR to detect pathogenic Leptospira species in kidney tissue.

@article{Fearnley2008TheDO,
  title={The development of a real-time PCR to detect pathogenic Leptospira species in kidney tissue.},
  author={Catherine Fearnley and Philip R. Wakeley and J Gallego-Beltran and Caleb Dalley and Sally J.M. Williamson and Catronia Gaudie and Martin John Woodward},
  journal={Research in veterinary science},
  year={2008},
  volume={85 1},
  pages={
          8-16
        }
}
A LightCycler real-time PCR hybridization probe-based assay that detects a conserved region of the16S rRNA gene of pathogenic but not saprophytic Leptospira species was developed for the rapid detection of pathogenic leptospires directly from processed tissue samples. In addition, a differential PCR specific for saprophytic leptospires and a control PCR targeting the porcine beta-actin gene were developed. To assess the suitability of these PCR methods for diagnosis, a trial was performed on… CONTINUE READING
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