The development of a quantitative PCR ELISA to determine HCMV DNAaemia levels in heart, heart/lung and lung transplant recipients.

@article{Barber1999TheDO,
  title={The development of a quantitative PCR ELISA to determine HCMV DNAaemia levels in heart, heart/lung and lung transplant recipients.},
  author={Lesley Barber and Jim J. J. Egan and Andrew J. Turner and Malcolm Guiver and A. A. Woodcock and Nizar N Yonan and Abdul K Deiraniya and Andrew J. Fox},
  journal={Journal of virological methods},
  year={1999},
  volume={82 1},
  pages={85-97}
}
The adaptation of the polymerase chain reaction (PCR) enzyme-linked immunosorbent assay (ELISA) using a co-amplified DNA standard to quantitate the human cytomegalovirus (HCMV) glycoprotein B (gB) gene in clinical samples is described. The PCR ELISA is a solution hybridisation system with colorimetric end stage detection of amplicons. A DNA internal standard (IS) was designed by replacing a probe sequence used currently within the gB region with a heterogeneous sequence, allowing co… CONTINUE READING