The development of a homologous transformation system for Aspergillus oryzae based on the nitrate assimilation pathway: A convenient and general selection system for filamentous fungal transformation

@article{Unkles2004TheDO,
  title={The development of a homologous transformation system for Aspergillus oryzae based on the nitrate assimilation pathway: A convenient and general selection system for filamentous fungal transformation},
  author={Shiela E. Unkles and Edward I. Campbell and Yolanda M. J. T. de Ruiter-Jacobs and Martien Broekhuijsen and Janet A. Macro and Dirk Carrez and Roland Contreras and Cees A. M. J. J. van den Hondel and James Robertson Kinghorn},
  journal={Molecular and General Genetics MGG},
  year={2004},
  volume={218},
  pages={99-104}
}
The development of an efficient and homologous transformation system for Aspergillus oryzae is described. This is based on nitrate reductase (niaD) of the nitrate assimilation pathway. The niaD system offers a number of inherent advantages over many other systems and may be of general use for nitrate-utilising filamentous fungi. Transformation frequencies of up to 800 transformants per microgram DNA are observed with A. oryzae. The preponderance of integration events take place at the resident… CONTINUE READING