The development of a continuous isothermal titration calorimetric method for equilibrium studies.

@article{Markova2004TheDO,
  title={The development of a continuous isothermal titration calorimetric method for equilibrium studies.},
  author={Natalia Markova and Dan Hall{\'e}n},
  journal={Analytical biochemistry},
  year={2004},
  volume={331 1},
  pages={77-88}
}
A continuous isothermal titration calorimetry (cITC) method for microcalorimeters has been developed. The method is based on continuous slow injection of a titrant into the calorimetric vessel. The experimental time for a cITC binding experiment is 12-20 min and the number of data points obtained is on the order of 1000. This gives an advantage over classical isothermal titration calorimetry (ITC) binding experiments that need 60-180 min to generate 20-30 data points. The method was validated… CONTINUE READING

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