The development of EST-SSR markers in Lilium regale and their cross-amplification in related species

@article{Yuan2012TheDO,
  title={The development of EST-SSR markers in Lilium regale and their cross-amplification in related species},
  author={Suxia Yuan and Liang Ge and Chun Liu and Jun Ming},
  journal={Euphytica},
  year={2012},
  volume={189},
  pages={393-419}
}
Simple-sequence repeat (SSR) markers are a common choice for assaying genetic diversity and genetic mapping due to their highly polymorphic and co-dominant nature. In this paper, we developed SSR markers derived from expressed sequence tags (EST-SSRs) based on transcriptome sequences in Lilium regale. In total, 59,046 UniGene sequences (approximately 27.37 Mb) were evaluated, where 1,716 SSR sites in the 1,606 UniGene sequences were identified by data mining. Of these SSRs, the trinucleotide… CONTINUE READING
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