The determination of five steroids in avian plasma by radioimmunoassay and competitive protein-binding

@article{Wingfield1975TheDO,
  title={The determination of five steroids in avian plasma by radioimmunoassay and competitive protein-binding},
  author={J. Wingfield and D. Farner},
  journal={Steroids},
  year={1975},
  volume={26},
  pages={311-327}
}
A method has been developed for the simultaneous determination of testosterone, 5alpha-dihydrotestosterone and corticosterone, or of estrone, estradiol-17beta and corticosterone, after separation on a Celite:propylene glycol:ethylene glycol column (6:1.5:1.5 w/v/v). The lower quarter of the column was packed with a Celite: water mixture (3:1 w/v) as a stationary phase (glycol) 'trap'. This effectively prevented leaching of the glycols into the eluate as the concentration of ethyl acetate in the… Expand
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