The detection of oseltamivir-resistant pandemic influenza A/H1N1 2009 viruses using a real-time RT-PCR assay.

@article{Chidlow2010TheDO,
  title={The detection of oseltamivir-resistant pandemic influenza A/H1N1 2009 viruses using a real-time RT-PCR assay.},
  author={Glenys R. Chidlow and Gerald B. Harnett and Simon Hedley Williams and Simone Sara Tempone and David John Speers and Aeron C. Hurt and Y Deng and David W Smith},
  journal={Journal of virological methods},
  year={2010},
  volume={169 1},
  pages={47-51}
}
A real-time reverse transcription PCR (rRT-PCR) assay was designed and evaluated for the detection of the point mutation in the influenza A N1 neuraminidase gene that results in a tyrosine to histidine substitution at amino acid position 275 (H275Y) causing resistance to oseltamivir, an antiviral neuraminidase inhibitor. The rRT-PCR assays detected the presence or absence of the H275Y mutation in 387/388 (99.7%) of clinical samples containing the pandemic influenza A/H1N1 2009 virus. The H275Y… CONTINUE READING

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Evaluation of a rapid molecular algorithm for detection of pandemic influenza A (H1N1) 2009 virus and screening for a key oseltamivir resistance (H275Y) substitution in neuraminidase. J

  • E. van der Vries, M. Jonges, +12 authors M. Schutten
  • 2010
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