The detection of mast cell subpopulations in formalin-fixed human tissues using a new monoclonal antibody specific for chymase.

@article{Buckley1999TheDO,
  title={The detection of mast cell subpopulations in formalin-fixed human tissues using a new monoclonal antibody specific for chymase.},
  author={Mark G. Buckley and Alan R. Mceuen and A F Walls},
  journal={The Journal of pathology},
  year={1999},
  volume={189 1},
  pages={138-43}
}
Chymase is an important marker for human mast cells as well as a mediator of inflammation and matrix remodelling, but research into chymase-containing mast cell subpopulations has been hampered by the lack of reagents suitable for use with formalin-fixed tissue. A monoclonal antibody to chymase (designated CC1) was prepared by immunizing a mouse with chymase purified from human skin, fusing the splenocytes with NS-1 myeloma cells, and screening the hybridoma supernatants by ELISA with… CONTINUE READING