The detection of human sapoviruses with universal and genogroup-specific primers

@article{Okada2006TheDO,
  title={The detection of human sapoviruses with universal and genogroup-specific primers},
  author={Mineyuki Okada and Yasutaka Yamashita and Mitsuaki Oseto and Kazuo Shinozaki},
  journal={Archives of Virology},
  year={2006},
  volume={151},
  pages={2503-2509}
}
Sapovirus (SV) causes gastroenteritis in humans and comprises genetically divergent viruses. A nested reverse transcription-polymerase chain reaction (RT-PCR) targeting the capsid-protein-coding region was developed using universal and genogroup-specific primer sets. The universal primers were capable of detecting human SV genogroups I, II, IV and V. Genetic analysis of the amplified products enabled us to phylogenetically determine the genotypes of the viruses. In addition, genogroup-specific… CONTINUE READING

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