The detection and quantitation of free desmosine and isodesmosine in human urine and their peptide-bound forms in sputum.

@article{Ma2003TheDA,
  title={The detection and quantitation of free desmosine and isodesmosine in human urine and their peptide-bound forms in sputum.},
  author={Shuren Ma and Seymour Lieberman and Gerard M. Turino and Yong Yoo Lin},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={2003},
  volume={100 22},
  pages={12941-3}
}
Desmosine (D) and isodesmosine (I), the intramolecular crosslinking amino acids that occur in chains of elastin, have now been found in free form in human urine. Until now, these amino acids (M(r) = 526) were found to occur in urine only as higher molecular weight (M (r) = 1,000-1,500) peptides. Thus, the previously used analytical methods required, as the first step, acid hydrolysis of the urine at elevated temperature to liberate D and I from their peptides. The analytical method described… CONTINUE READING