The design and validation of a novel phenotypic assay to determine HIV-1 coreceptor usage of clinical isolates.

@article{Lin2010TheDA,
  title={The design and validation of a novel phenotypic assay to determine HIV-1 coreceptor usage of clinical isolates.},
  author={Nina H. Lin and Daniel M Negusse and Rameen Beroukhim and Francoise F. Giguel and Shahin Lockman and Myron E Essex and Daniel R. Kuritzkes},
  journal={Journal of virological methods},
  year={2010},
  volume={169 1},
  pages={39-46}
}
A phenotypic assay to determine coreceptor usage of HIV-1 has been developed for rapid testing of clinical samples. The assay is based on the synthesis of viral stock from full-length env amplicons isolated from patient's plasma. Pseudoviral stock is generated rapidly by using an overlapping PCR method to assemble a CMV promoter to env, followed by co-transfection into producer cells with a HIV plasmid (pNL4-3.Luc.R(-)E(-)) containing a non-functional env. The coreceptor used by the viral… CONTINUE READING
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