The design, expression, and characterization of human insulin-like growth factor II (IGF-II) mutants specific for either the IGF-II/cation-independent mannose 6-phosphate receptor or IGF-I receptor.

@article{Sakano1991TheDE,
  title={The design, expression, and characterization of human insulin-like growth factor II (IGF-II) mutants specific for either the IGF-II/cation-independent mannose 6-phosphate receptor or IGF-I receptor.},
  author={K i Sakano and T Enjoh and F Numata and Hironobu Fujiwara and Yasumasa Marumoto and N Higashihashi and Yuichiro Z Sato and James F. Perdue and Yoko Fujita-Yamaguchi},
  journal={The Journal of biological chemistry},
  year={1991},
  volume={266 31},
  pages={20626-35}
}
Five mutants of recombinant insulin-like growth factor-II (rIGF-II) that bound with high affinity to either the IGF-II/cation-independent mannose 6-phosphate (IGF-II/CIM6-P) or the IGF-I receptor were prepared by site-directed mutagenic procedures, expressed as fusion proteins in the larva of Bombyx mori or Escherichia coli, purified to homogeneity, renatured, and characterized in terms of their receptor binding affinities and specificities as well as their biological activities. Class I… CONTINUE READING