The dependence of cis-diamminedichloroplatinum(II) binding to DNA upon the GC content: a thermal and spectrophotometrical investigation

@inproceedings{Haroutiunian1991TheDO,
  title={The dependence of cis-diamminedichloroplatinum(II) binding to DNA upon the GC content: a thermal and spectrophotometrical investigation},
  author={Samvel G Haroutiunian and Bruno Bruni and Roberto Monnanni and Pierluigi Orioli and Stefano Mangani},
  year={1991}
}
Abstract The binding of cis -diamminedichloroplatinum(II) ( cis -DDP) to DNA is investigated as a function of the GC content and of the cis -DDP concentration. Analysis of the melting curves and of the UV and CD spectra shows that binding of cis -DDP to DNA is highly sequence specific, GC rich DNAs being destabilized more effectively. At low levels of platination, stabilization of GC- and destabilization of AT-base pairs can be noted. In AT rich DNA, cis - DDP binding favours the B→ A… CONTINUE READING