The demethylation of guaiacol by a new bacterial cytochrome P-450.

  title={The demethylation of guaiacol by a new bacterial cytochrome P-450.},
  author={A Dardas and Dezso G{\'a}l and Michel Barrelle and G. Sauret-Ignazi and R Sterjiades and Jean Pelmont},
  journal={Archives of biochemistry and biophysics},
  volume={236 2},
Spectroscopic studies were carried with a cytochrome P-450 in Moraxella sp., strain GU2, that could grow on guaiacol or 2-ethoxyphenol as the sole source of carbon and energy. The dissociation constant of the guaiacol-cytochrome complex was estimated to 0.15 microM, as determined in vivo or using the cell soluble extract. Cytochrome P-450 could also bind 2-ethoxyphenol, 2-propoxyphenol, and 2-butoxyphenol, and the dissociation constants have been determined in each case. Metyrapone depressed… CONTINUE READING

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