The delta-opioid receptor: isolation of a cDNA by expression cloning and pharmacological characterization.

@article{Kieffer1992TheDR,
  title={The delta-opioid receptor: isolation of a cDNA by expression cloning and pharmacological characterization.},
  author={Brigitte Lina Kieffer and Katia Befort and Claire Gav{\'e}riaux-Ruff and Ch. Hirth},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1992},
  volume={91 3},
  pages={1193}
}
A random primed expression cDNA library was constructed from the RNA of NG 108-15 cells. Pools of plasmid DNA were transfected into COS cells, which were screened for their ability to bind 3H-labeled Tyr-D-Thr-Gly-Phe-Leu-Thr, a tritiated agonist for the delta-opioid receptor. A cDNA was isolated that encodes a 371-amino acid-residue protein presenting all the structural characteristics of receptors that interact with guanine nucleotide-binding proteins. Noticeable features are (i) the high… CONTINUE READING

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When expressed in COS cells , the receptor exhibits pharmacological properties similar to those of the native receptor : high - affinity binding sites for 3H - labeled Tyr - D - Thr - Gly - Phe - Leu - Thr ( Kd = 1.4 nM ) , stereospecific binding sites for the - enantiomers of levorphanol and naloxone , and the selectivity profile of a delta receptor , as determined by competition experiments with a set of mu- , delta- , and kappa - opioid ligands .
Pools of plasmid DNA were transfected into COS cells , which were screened for their ability to bind 3H - labeled Tyr - D - Thr - Gly - Phe - Leu - Thr , a tritiated agonist for the delta - opioid receptor .
When expressed in COS cells , the receptor exhibits pharmacological properties similar to those of the native receptor : high - affinity binding sites for 3H - labeled Tyr - D - Thr - Gly - Phe - Leu - Thr ( Kd = 1.4 nM ) , stereospecific binding sites for the - enantiomers of levorphanol and naloxone , and the selectivity profile of a delta receptor , as determined by competition experiments with a set of mu- , delta- , and kappa - opioid ligands .
Pools of plasmid DNA were transfected into COS cells , which were screened for their ability to bind 3H - labeled Tyr - D - Thr - Gly - Phe - Leu - Thr , a tritiated agonist for the delta - opioid receptor .
When expressed in COS cells , the receptor exhibits pharmacological properties similar to those of the native receptor : high - affinity binding sites for 3H - labeled Tyr - D - Thr - Gly - Phe - Leu - Thr ( Kd = 1.4 nM ) , stereospecific binding sites for the - enantiomers of levorphanol and naloxone , and the selectivity profile of a delta receptor , as determined by competition experiments with a set of mu- , delta- , and kappa - opioid ligands .
When expressed in COS cells , the receptor exhibits pharmacological properties similar to those of the native receptor : high - affinity binding sites for 3H - labeled Tyr - D - Thr - Gly - Phe - Leu - Thr ( Kd = 1.4 nM ) , stereospecific binding sites for the - enantiomers of levorphanol and naloxone , and the selectivity profile of a delta receptor , as determined by competition experiments with a set of mu- , delta- , and kappa - opioid ligands .
Pools of plasmid DNA were transfected into COS cells , which were screened for their ability to bind 3H - labeled Tyr - D - Thr - Gly - Phe - Leu - Thr , a tritiated agonist for the delta - opioid receptor .
Pools of plasmid DNA were transfected into COS cells , which were screened for their ability to bind 3H - labeled Tyr - D - Thr - Gly - Phe - Leu - Thr , a tritiated agonist for the delta - opioid receptor .
Pools of plasmid DNA were transfected into COS cells , which were screened for their ability to bind 3H - labeled Tyr - D - Thr - Gly - Phe - Leu - Thr , a tritiated agonist for the delta - opioid receptor .
Pools of plasmid DNA were transfected into COS cells , which were screened for their ability to bind 3H - labeled Tyr - D - Thr - Gly - Phe - Leu - Thr , a tritiated agonist for the delta - opioid receptor .
When expressed in COS cells , the receptor exhibits pharmacological properties similar to those of the native receptor : high - affinity binding sites for 3H - labeled Tyr - D - Thr - Gly - Phe - Leu - Thr ( Kd = 1.4 nM ) , stereospecific binding sites for the - enantiomers of levorphanol and naloxone , and the selectivity profile of a delta receptor , as determined by competition experiments with a set of mu- , delta- , and kappa - opioid ligands .
When expressed in COS cells , the receptor exhibits pharmacological properties similar to those of the native receptor : high - affinity binding sites for 3H - labeled Tyr - D - Thr - Gly - Phe - Leu - Thr ( Kd = 1.4 nM ) , stereospecific binding sites for the - enantiomers of levorphanol and naloxone , and the selectivity profile of a delta receptor , as determined by competition experiments with a set of mu- , delta- , and kappa - opioid ligands .
When expressed in COS cells , the receptor exhibits pharmacological properties similar to those of the native receptor : high - affinity binding sites for 3H - labeled Tyr - D - Thr - Gly - Phe - Leu - Thr ( Kd = 1.4 nM ) , stereospecific binding sites for the - enantiomers of levorphanol and naloxone , and the selectivity profile of a delta receptor , as determined by competition experiments with a set of mu- , delta- , and kappa - opioid ligands .
When expressed in COS cells , the receptor exhibits pharmacological properties similar to those of the native receptor : high - affinity binding sites for 3H - labeled Tyr - D - Thr - Gly - Phe - Leu - Thr ( Kd = 1.4 nM ) , stereospecific binding sites for the - enantiomers of levorphanol and naloxone , and the selectivity profile of a delta receptor , as determined by competition experiments with a set of mu- , delta- , and kappa - opioid ligands .
When expressed in COS cells , the receptor exhibits pharmacological properties similar to those of the native receptor : high - affinity binding sites for 3H - labeled Tyr - D - Thr - Gly - Phe - Leu - Thr ( Kd = 1.4 nM ) , stereospecific binding sites for the - enantiomers of levorphanol and naloxone , and the selectivity profile of a delta receptor , as determined by competition experiments with a set of mu- , delta- , and kappa - opioid ligands .
When expressed in COS cells , the receptor exhibits pharmacological properties similar to those of the native receptor : high - affinity binding sites for 3H - labeled Tyr - D - Thr - Gly - Phe - Leu - Thr ( Kd = 1.4 nM ) , stereospecific binding sites for the - enantiomers of levorphanol and naloxone , and the selectivity profile of a delta receptor , as determined by competition experiments with a set of mu- , delta- , and kappa - opioid ligands .
Pools of plasmid DNA were transfected into COS cells , which were screened for their ability to bind 3H - labeled Tyr - D - Thr - Gly - Phe - Leu - Thr , a tritiated agonist for the delta - opioid receptor .
Pools of plasmid DNA were transfected into COS cells , which were screened for their ability to bind 3H - labeled Tyr - D - Thr - Gly - Phe - Leu - Thr , a tritiated agonist for the delta - opioid receptor .
Pools of plasmid DNA were transfected into COS cells , which were screened for their ability to bind 3H - labeled Tyr - D - Thr - Gly - Phe - Leu - Thr , a tritiated agonist for the delta - opioid receptor .
Pools of plasmid DNA were transfected into COS cells , which were screened for their ability to bind 3H - labeled Tyr - D - Thr - Gly - Phe - Leu - Thr , a tritiated agonist for the delta - opioid receptor .
When expressed in COS cells , the receptor exhibits pharmacological properties similar to those of the native receptor : high - affinity binding sites for 3H - labeled Tyr - D - Thr - Gly - Phe - Leu - Thr ( Kd = 1.4 nM ) , stereospecific binding sites for the - enantiomers of levorphanol and naloxone , and the selectivity profile of a delta receptor , as determined by competition experiments with a set of mu- , delta- , and kappa - opioid ligands .
When expressed in COS cells , the receptor exhibits pharmacological properties similar to those of the native receptor : high - affinity binding sites for 3H - labeled Tyr - D - Thr - Gly - Phe - Leu - Thr ( Kd = 1.4 nM ) , stereospecific binding sites for the - enantiomers of levorphanol and naloxone , and the selectivity profile of a delta receptor , as determined by competition experiments with a set of mu- , delta- , and kappa - opioid ligands .
When expressed in COS cells , the receptor exhibits pharmacological properties similar to those of the native receptor : high - affinity binding sites for 3H - labeled Tyr - D - Thr - Gly - Phe - Leu - Thr ( Kd = 1.4 nM ) , stereospecific binding sites for the - enantiomers of levorphanol and naloxone , and the selectivity profile of a delta receptor , as determined by competition experiments with a set of mu- , delta- , and kappa - opioid ligands .
When expressed in COS cells , the receptor exhibits pharmacological properties similar to those of the native receptor : high - affinity binding sites for 3H - labeled Tyr - D - Thr - Gly - Phe - Leu - Thr ( Kd = 1.4 nM ) , stereospecific binding sites for the - enantiomers of levorphanol and naloxone , and the selectivity profile of a delta receptor , as determined by competition experiments with a set of mu- , delta- , and kappa - opioid ligands .
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