The delta- and epsilon-subunits of bovine F1-ATPase interact to form a heterodimeric subcomplex.

The delta-subunit of bovine F1-ATPase was expressed from a bacterial vector at fairly high level in Escherichia coli, but the yield of bovine epsilon-subunit was rather low under similar conditions. However, co-expression of the proteins from a dicistronic operon delta-epsilon in the same expression vector, produced both of them in good yield in a soluble… CONTINUE READING