The dark side of green fluorescent protein.

  title={The dark side of green fluorescent protein.},
  author={Xin Zhou and Ra{\'u}l Carranco and Stanislav Vitha and Timothy C. Hall},
  journal={The New phytologist},
  volume={168 2},
Here, severe interference of chlorophyll with green fluorescent protein (GFP) fluorescence is described for medicago (Medicago truncatula), rice (Oryza sativa) and arabidopsis (Arabidopsis thaliana). This interference disrupts the proportional relationship between GFP content and fluorescence that is intrinsic to its use as a quantitative reporter. The involvement of chlorophyll in the loss of GFP fluorescence with leaf age was shown in vivo, by the removal of chlorophyll through etiolation or… CONTINUE READING

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