The cytotoxic cell protease granzyme B initiates apoptosis in a cell-free system by proteolytic processing and activation of the ICE/CED-3 family protease, CPP32, via a novel two-step mechanism.

@article{Martin1996TheCC,
  title={The cytotoxic cell protease granzyme B initiates apoptosis in a cell-free system by proteolytic processing and activation of the ICE/CED-3 family protease, CPP32, via a novel two-step mechanism.},
  author={S J Martin and Gustavo Pessini Amarante-Mendes and Lianfa F. Shi and T H Chuang and Carlos A Casiano and Glennys A O'Brien and Patrick O Fitzgerald and Eng M Tan and Gary M. Bokoch and Arnold H. Greenberg and Douglas R Green},
  journal={The EMBO journal},
  year={1996},
  volume={15 10},
  pages={2407-16}
}
The major mechanism of cytotoxic lymphocyte killing involves the directed release of granules containing perforin and a number of proteases onto the target cell membrane. One of these proteases, granzyme B, has an unusual substrate site preference for Asp residues, a property that it shares with members of the emerging interleukin-1beta-converting enzyme (ICE)/CED-3 family of proteases. Here we show that granzyme B is sufficient to reproduce rapidly all of the key features of apoptosis… CONTINUE READING

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