The cytosolic and glycosomal glyceraldehyde-3-phosphate dehydrogenase from Trypanosoma brucei. Kinetic properties and comparison with homologous enzymes.

@article{Lambeir1991TheCA,
  title={The cytosolic and glycosomal glyceraldehyde-3-phosphate dehydrogenase from Trypanosoma brucei. Kinetic properties and comparison with homologous enzymes.},
  author={Anne Marie Lambeir and A. M. Loiseau and Douglas A. Kuntz and Fr{\'e}d{\'e}ric M. D. Vellieux and Paul A. M. Michels and Fred R. Opperdoes},
  journal={European journal of biochemistry},
  year={1991},
  volume={198 2},
  pages={429-35}
}
The protozoan haemoflagellate Trypanosoma brucei has two NAD-dependent glyceraldehyde-3-phosphate dehydrogenase isoenzymes, each with a different localization within the cell. One isoenzyme is found in the cytosol, as in other eukaryotes, while the other is found in the glycosome, a microbody-like organelle that fulfils an essential role in glycolysis. The kinetic properties of the purified glycosomal and cytosolic isoenzymes were compared with homologous enzymes from other organisms. Both… CONTINUE READING