The cysteine-cluster motif of c-Src: its role for the heavy metal-mediated activation of kinase.

@article{Senga2008TheCM,
  title={The cysteine-cluster motif of c-Src: its role for the heavy metal-mediated activation of kinase.},
  author={Takeshi Senga and Hitoki Hitoki Hasegawa and Miwa Tanaka and Mohammad Aminur Rahman and Satoko Ito and Michinari Hamaguchi},
  journal={Cancer science},
  year={2008},
  volume={99 3},
  pages={571-5}
}
We have previously reported that c-Src is activated by mercuric chloride (HgCl(2)). We investigated the mechanism of this activation and found that in vitro activation of c-Src by HgCl(2) did not require tyrosine residues at 416 and 527. Both SH2 and SH3 domains of c-Src were dispensable for the activation by HgCl(2). In contrast, iodoacetoamide (IAA) that binds to thiol side chain of cysteine blocked the activation of c-Src by HgCl(2). To obtain more clues, each cysteine residue of c-Src was… CONTINUE READING