The cystathionase-rhodanese system.

@article{Szczepkowski1967TheCS,
  title={The cystathionase-rhodanese system.},
  author={T. Szczepkowski and J. L. Wood},
  journal={Biochimica et biophysica acta},
  year={1967},
  volume={139 2},
  pages={
          469-78
        }
}
Abstract Cystathionase ( l -homoserine hydrolase (deaminating), EC 4.2.1.15) and rhodanese ∗∗ (thiosulfate: cyanide sulfurtransferase, EC 2.8.1.1) have been combined to form a coupled enzyme system which is capable of utilizing cysteine sulfur for transsulfuration. The initial product of the action of Cystathionase on cystine, thiocysteine, is, at least in part, converted to bis-(2-amino-2-carboxyethyl) trisulfide, “thiocystine”. Rhodanese accepts the products of the action of cystathionase… Expand
Transfer of persulfide sulfur from thiocystine to rhodanese.
TLDR
Thiocystine and thiosulfate both produce a rhodanese persulfide as a stable intermediate in sulfur transfer, and only the sulfane sulfur of 35S-labeled thiocyStine was transferred to r Rhodanese. Expand
Biochemistry Of Thiocystine
Thiocystine, bis-[2-amino-2-carboxyethyl]trisulfide, is a relatively stable trisulfide analog of cystine. The compound was first isolated by Fletcher and Robson1 from hydrochloric acid hydrolysatesExpand
γ-Liaza cystationinowa Cystathionine γ-lyase
γ-Cystathionase (CTH, EC: 4.4.1.1), an enzyme widely distributed in the world of prokaryotic and eukaryotic organisms, catalyzes the formation and transformations of sulfane sulfur-containingExpand
Metabolism of L-[sulfane-34S]thiocystine by Escherichia coli.
  • R. White
  • Chemistry, Medicine
  • Biochemistry
  • 1982
TLDR
The results suggest that the first step in the metabolism of thiocystine by E. coli involves the beta elimination of pyruvate, characteristic of the cleavage reactions catalyzed by beta-cystathionase. Expand
In vivo detoxification of cyanide by cystathionase γ-lyase
Abstract The results of several in vitro studies have suggested that the enzyme cystathionase γ-lyase (EC 4.4.1.1) may function in the endogenous detoxification of cyanide; however, this possibilityExpand
Persulfide properties of thiocystine and related trisulfides
Abstract Thiocystine (bis[2-amino-2-carboxyethyl]trisulfide) functions as a persulfide in transferring its sulfane sulfur to thiophilic acceptors. This occurs by formation of a reactive intermediate,Expand
Cysteine thiosulfonate in cystine metabolism.
Abstract A sulfur-containing amino acid was observed in mammalian cystine metabolism, in vitro and in vivo , which we have characterized as 2-amino, 3-(thio-thiosulfonate)propionic acid (cysteineExpand
Sulfurtransferase Enzymes Involved in Cyanide Metabolism
The major route of cyanide metabolism is conversion to thiocyanate, a nontoxic metabolite that is excreted. The sulfurtransferase enzymes, rhodanese and 3-mercaptopyruvate sulfurtransferase (MST),Expand
Unraveling the role of thiosulfate sulfurtransferase in metabolic diseases.
TLDR
This review summarizes research related to the enzymatic properties and functions of TST, to then explore the association between the effects of T ST on mitochondria and development of diseases such as diabetes and obesity. Expand
CONVERSION OF CYSTINE OR CYSTEINE TO THE ANTIBACTERIAL COMPOUND BIS-(2-AMINO-2-CARBOXYETHYL) TRISULFIDE (BACTIN) ENHANCED BY FERRIC CHLORIDE AND SODIUM NITRITE
Heating of cystine at pH 6 or in hydrochloric acid, yielded Bis-(2-amino-2-carboxyethyl) trisulfide (Bactin), which had a bacteriostatic action on Lactobacillus plantarum. Heating of cysteine did notExpand
...
1
2
3
4
5
...

References

SHOWING 1-10 OF 19 REFERENCES
A crystalline enzyme that cleaves homoserine and cystathionine. II. Prosthetic group.
TLDR
The present paper deals with resolution of the enzyme into pyridoxal-5-P and catalytically inactive apoenzyme, and the subsequent reconstitution of the active enzyme by combining the two components, thus establishing that pyridine5-phosphate is the coenzyme of this enzyme. Expand
Microbial transsulfuration: the mechanism of an enzymatic disulfide elimination reaction.
  • M. Flavin
  • Medicine, Biology
  • The Journal of biological chemistry
  • 1962
TLDR
The present evidence suggests that wild type Neurospora may contain only one cleavage enzyme, which catalyzes a heterogeneous decomposition of cystathionine. Expand
Detoxication of cyanide by cystine.
TLDR
Radioactivity measurements showed that the iminothiazolidinecarboxylic acid was produced from cystine, while the thiocyanate chiefly was formed from other sources of sulfur, which constitutes an independent pathway for detoxi- cabion of cyanide. Expand
Tissue sulfhydryl groups.
  • G. Ellman
  • Chemistry, Medicine
  • Archives of biochemistry and biophysics
  • 1959
Abstract A water-soluble (at pH 8) aromatic disulfide [5,5′-dithiobis(2-nitrobenzoic acid)] has been synthesized and shown to be useful for determination of sulfhydryl groups. Several applicationsExpand
Further evidence on the identity of cystathionase and cysteine desulfhydrase.
...
1
2
...