The cycHJKL genes of Rhizobium meliloti involved in cytochrome c biogenesis are required for “respiratory” nitrate reduction ex planta and for nitrogen fixation during symbiosis

@article{Kereszt1995TheCG,
  title={The cycHJKL genes of Rhizobium meliloti involved in cytochrome c biogenesis are required for “respiratory” nitrate reduction ex planta and for nitrogen fixation during symbiosis},
  author={Attila Kereszt and Krystyna Ślaska-Kiss and P{\'e}ter Putnoky and Zs{\'o}fia B{\'a}nfalvi and Ad{\'a}m Kondorosi},
  journal={Molecular and General Genetics MGG},
  year={1995},
  volume={247},
  pages={39-47}
}
We report the genetic and biochemical analysis of Rhizobium meliloti mutants defective in symbiotic nitrogen fixation (Fix−) and “respiratory” nitrate reduction (Rnr−). The mutations were mapped close to the ade-1 and cys-46 chromosomal markers and the mutated locus proved to be identical to the previously described fix-14 locus. By directed Tn5 mutagenesis, a 4.5 kb segment of the chromosome was delimited in which all mutations resulted in Rnr− and Fix− phenotypes. Nucleotide sequence analysis… CONTINUE READING

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