The current status of primary hepatocyte culture

@article{Mitaka1998TheCS,
  title={The current status of primary hepatocyte culture},
  author={Toshihiro Mitaka},
  journal={International Journal of Experimental Pathology},
  year={1998},
  volume={79}
}
  • T. Mitaka
  • Published 1998
  • Biology, Medicine
  • International Journal of Experimental Pathology
Recently, there have been significant advances toward the development of culture conditions that promote proliferation of primary rodent hepatocytes. There are two major methods for the multiplication of hepatocytes in vitro: one is the use of nicotinamide, the other is the use of a nutrient‐rich medium. In the medium containing a high concentration of nicotinamide and a growth factor, primary hepatocytes can proliferate well. In this culture condition small mononucleate cells, which are named… Expand
Small hepatocytes in primary cultures
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References

SHOWING 1-10 OF 214 REFERENCES
Chapter 31 Primary and Long-Term Culture of Adult Rat Liver Epithelial Cells
TLDR
Assessment of the behavior of representative hepatocytes in cultures initiated from high-yield perfusion dissociates permits for the first time assessment of the ability of hepatocytes to adapt to culture and to proliferate. Expand
Growth and maturation of small hepatocytes isolated from adult rat liver.
TLDR
The small hepatocytes may be "committed progenitor cells" which can further differentiate into mature hepatocytes that had a large cytoplasm and sometimes two nuclei and the secretion of albumin in the medium by the hepatocytes increased with time in culture. Expand
Long-term cultivation of adult rat hepatocytes that undergo multiple cell divisions and express normal parenchymal phenotypes.
TLDR
The culture medium used also supported the growth of stellate cells (Ito cells) that had contaminated the original preparation in small numbers and seems to cooperatively stimulate a proliferative population of hepatocytes. Expand
Growth and differentiation in culture of clonogenic hepatocytes that express both phenotypes of hepatocytes and biliary epithelial cells.
TLDR
A cell fraction containing small hepatocytes and nonparenchymal cells was isolated from the adult rat liver and was cultured in the presence of vitamin C to contain liver progenitor-like cells that can differentiate during culture into cells expressing phenotypes of mature hepatocytes or biliary epithelial cells. Expand
GROWTH CONTROL OF DIFFERENTIATED ADULT RAT HEPATOCYTES IN PRIMARY CULTURE *
TLDR
The properties of “normal” adult hepatocytes cultured in this laboratory will be reviewed and new observations linking early ionic signalling events with “membrane potential” and “intracellular pH” changes will be described. Expand
Small cell colonies appear in the primary culture of adult rat hepatocytes in the presence of nicotinamide and epidermal growth factor
TLDR
Colonies of small hepatocytes appeared after the culture of primary adult rat hepatocytes for 4 days in serum‐free modified Dulbecco's modified Eagle's medium containing 10 mmol/L nicotinamide and 10 ng/ml epidermal growth factor, indicating that they were derived from hepatocytes. Expand
Characteristics of small cell colonies developing in primary cultures of adult rat hepatocytes
SummaryPhenotypes of the cells developing into small colonies after days of primary culture of adult rat hepatocytes in serum-free modified Dulbecco Modified Eagles’ medium containing 10 mMExpand
Effect of age on the formation of small-cell colonies in cultures of primary rat hepatocytes.
TLDR
The proliferation of primary cultured rat hepatocytes was observed in serum-free modified Dulbecco's modified Eagle's medium supplemented with 10 mM nicotinamide and 10 ng/ml of epidermal growth factor and suggests that the cells in the small-cell colonies were derived from hepatocytes. Expand
Exposure of primary rat hepatocytes in long‐term DMSO culture to selected transition metals induces hepatocyte proliferation and formation of duct‐like structures
TLDR
It is concluded that under these specific nutritive conditions, primary rat hepatocytes proliferate and, with time, begin to form duct‐like structures with altered gene expression and ultrastructural properties. Expand
Nicotinamide prolongs survival of primary cultured hepatocytes without involving loss of hepatocyte-specific functions.
TLDR
The results suggest that the maintenance of the intracellular NAD level is essential for the growth and functioning of hepatocytes and that nicotinamide can preserve the NAD level by blocking NAD degradation as well as by acting as a precursor for NAD synthesis. Expand
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