The crystal structure of Rv0793, a hypothetical monooxygenase from M.␣tuberculosis

@article{Lemieux2005TheCS,
  title={The crystal structure of Rv0793, a hypothetical monooxygenase
from M.␣tuberculosis},
  author={M. Joanne Lemieux and Claire A Ference and Maia M. Cherney and Metian Wang and Craig R Garen and Michael N. G. James},
  journal={Journal of Structural and Functional Genomics},
  year={2005},
  volume={6},
  pages={245-257}
}
Mycobacterium tuberculosis infects millions worldwide. The Structural Genomics Consortium for M. tuberculosis has targeted all genes from this bacterium in hopes of discovering and developing new therapeutic agents. Open reading frame Rv0793 from M. tuberculosis was annotated with an unknown function. The 3-dimensional structure of Rv0793 has been solved to 1.6 Å resolution. Its structure is very similar to that of Streptomyces coelicolor ActVA-Orf6, a monooxygenase that participates in… Expand
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References

SHOWING 1-10 OF 34 REFERENCES
A New Family of Type III Polyketide Synthases in Mycobacterium tuberculosis*
TLDR
It is proposed that these proteins might have evolved by fusing the catalytic machinery of CHS and β-ketoacyl synthases, the two evolutionarily related members with conserved thiolase fold, to provide a fascinating example of metabolite divergence in CHS-like proteins. Expand
Identification of a monooxygenase from Streptomyces coelicolor A3(2) involved in biosynthesis of actinorhodin: purification and characterization of the recombinant enzyme
TLDR
Site-directed mutagenesis was used to investigate the role of histidine residues thought to be important in the reaction; mutants lacking His-52 displayed much-reduced activity, consistent with the proposed mechanistic hypothesis that this histidine acts as a general base during catalysis. Expand
The structure of ActVA‐Orf6, a novel type of monooxygenase involved in actinorhodin biosynthesis
TLDR
This is the first crystal structure of an enzyme involved in the tailoring of a type II aromatic polyketide and illustrates some of the enzyme–substrate recognition features that may apply to a range of other enzymes involved in modifying apolyketide core structure. Expand
A glycolipid of hypervirulent tuberculosis strains that inhibits the innate immune response
TLDR
The identification and functional relevance of a highly biologically active lipid species—a polyketide synthase-derived phenolic glycolipid (PGL) produced by a subset of M. tuberculosis isolates belonging to the W-Beijing family that show ‘hyperlethality’ in murine disease models are described. Expand
Structural and Biochemical Evidence for an Enzymatic Quinone Redox Cycle in Escherichia coli
  • M. Adams, Z. Jia
  • Chemistry, Medicine
  • Journal of Biological Chemistry
  • 2005
TLDR
The crystal structure of YgiN is presented, a protein of hitherto unknown function that was able to reoxidize menadiol, the product of the “modulator of drug activity B” (MdaB) enzymatic reaction, lending evidence to the possible existence of a novel quinone redox cycle in E. coli. Expand
Cloning, Sequencing, and Heterologous Expression of the elmGHIJ Genes Involved in the Biosynthesis of the Polyketide Antibiotic Elloramycin from Streptomyces olivaceus Tü2353
TLDR
These results provide direct biochemical evidence for the hypothesis that the biosynthesis of 1 in S. olivaceus parallels that of tetracenomycin C (2) in Streptomyces glaucescens and support the notion that the metabolism of the highly hydroxylated cyclohexenone moiety in other polyketides most likely follows the same paradigm as the tetracanomycin B2 or A... Expand
Unique Features of the sodC-encoded Superoxide Dismutase from Mycobacterium tuberculosis, a Fully Functional Copper-containing Enzyme Lacking Zinc in the Active Site*
TLDR
It is proposed that the observed structural rearrangements are required to build up a zinc-independent but fully active and stable copper-containing SOD. Expand
Crystal structure of bacterial multidrug efflux transporter AcrB
TLDR
The crystal structure of AcrB implies that substrates translocated from the cell interior through the transmembrane region and from the periplasm through the vestibules are collected in the central cavity and then actively transported through the pore into the TolC tunnel. Expand
Crystallization and preliminary X-ray diffraction studies of a monooxygenase from Streptomyces coelicolor A3(2) involved in the biosynthesis of the polyketide actinorhodin.
The aromatic monooxygenase ActVA-Orf6 from Streptomyces coelicolor A3(2) that catalyses an unusual oxidation on the actinorhodin biosynthetic pathway has been crystallized. The crystals diffract toExpand
The TB structural genomics consortium: a resource for Mycobacterium tuberculosis biology.
TLDR
This review describes the TB Structural Genomics Consortium and some of the proteins for which the Consortium is in the progress of determining three-dimensional structures. Expand
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