The critical role of the stem region as a functional domain responsible for the oligomerization and Golgi localization of N-acetylglucosaminyltransferase V. The involvement of a domain homophilic interaction.

@article{Sasai2001TheCR,
  title={The critical role of the stem region as a functional domain responsible for the oligomerization and Golgi localization of N-acetylglucosaminyltransferase V. The involvement of a domain homophilic interaction.},
  author={Ken Sasai and Yukio Ikeda and Takeo Tsuda and Hideshi Ihara and Hiroaki Korekane and Kunio Shiota and Naoyuki Taniguchi},
  journal={The Journal of biological chemistry},
  year={2001},
  volume={276 1},
  pages={759-65}
}
We demonstrated that a region in the stem of N-acetylglucosaminyltransferase V (GnT-V), a Golgi resident protein, is not required for enzyme activity but serves as functional domain, responsible for intracellular localization. Deletion of the domain led to complete retention of the kinetic properties but resulted in the cell surface localization of the enzyme as well as its efficient secretion into the medium. The lack of this domain concomitantly abolished the disulfide-mediated… CONTINUE READING