The covalent attachment of adhesion molecules to silicone membranes for cell stretching applications.

@article{Wipff2009TheCA,
  title={The covalent attachment of adhesion molecules to silicone membranes for cell stretching applications.},
  author={Pierre-Jean Wipff and Hicham Majd and Chitrangada Acharya and Lara Buscemi and Jean-Jacques Meister and Boris Hinz},
  journal={Biomaterials},
  year={2009},
  volume={30 9},
  pages={1781-9}
}
Strain devices with expandable polydimethylsiloxane (PDMS) culture membranes are frequently used to stretch cells in vitro, mimicking mechanically dynamic tissue environments. To immobilize cell-adhesive molecules to the otherwise non-adhesive PDMS substrate, hydrophobic, electrostatic and covalent surface coating procedures have been developed. The efficacy of different coating strategies to transmit stretches to cells however is poorly documented and has not been compared. We describe a novel… CONTINUE READING

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