The coordinate release of cytochrome c during apoptosis is rapid, complete and kinetically invariant

@article{Goldstein2000TheCR,
  title={The coordinate release of cytochrome c during apoptosis is rapid, complete and kinetically invariant},
  author={Joshua C. Goldstein and Nigel J. Waterhouse and P. Juin and Gerard I. Evan and Douglas R. Green},
  journal={Nature Cell Biology},
  year={2000},
  volume={2},
  pages={156-162}
}
Release of cytochrome c from mitochondria triggers activation of caspase proteases and death of a cell by apoptosis. However, the mechanism and kinetics of cytochrome c release remain unknown. Here we study this event by using green fluorescent protein (GFP)-tagged cytochrome c, and find that the release of cytochrome-c–GFP always precedes exposure of phosphatidylserine and the loss of plasma-membrane integrity — characteristics of apoptotic cells. Once initiated, the release of cytochrome-c… 
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TLDR
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TLDR
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TLDR
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TLDR
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Smac/DIABLO and cytochrome c are released from mitochondria through a similar mechanism during UV-induced apoptosis
TLDR
In HeLa cells, both Smac and Cyt-c are released from mitochondria during UV-induced apoptosis through the same permeability transition mechanism, which the authors believe is triggered by the aggregation of Bax in the outer mitochondrial membrane to form lipid-protein complex.
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