The construction of bifunctional fusion proteins consisting of MutS and GFP.

@article{StanisawskaSachadyn2006TheCO,
  title={The construction of bifunctional fusion proteins consisting of MutS and GFP.},
  author={Anna Stanisławska-Sachadyn and Paweł Sachadyn and Karolina Ihle and Cezary Sydorczuk and Katarzyna Wiejacha and J{\'o}zef W Kur},
  journal={Journal of biotechnology},
  year={2006},
  volume={121 2},
  pages={134-43}
}
MutS as a mismatch binding protein is a promising tool for SNP detection. Green fluorescent protein (GFP) is known as an excellent reporter domain. We constructed chimeric proteins consisting of MutS from Thermus thermophilus and GFPuv from Aequorea victoria by cloning the GFPuv gene into the plasmid vectors carrying the mutS gene. The GFPuv domain fused to… CONTINUE READING