The conjugative transposon Tn5397 has a strong preference for integration into its Clostridium difficile target site.

@article{Wang2006TheCT,
  title={The conjugative transposon Tn5397 has a strong preference for integration into its Clostridium difficile target site.},
  author={Hongmei Wang and Margaret C. M. Smith and Peter Mullany},
  journal={Journal of bacteriology},
  year={2006},
  volume={188 13},
  pages={4871-8}
}
Tn5397 is a conjugative transposon, originally isolated from Clostridium difficile. The Tn5397 transposase TndX is related to the phage-encoded serine integrases and the Clostridium perfringens Tn4451 transposase TnpX. TndX is required for the insertion and excision of the transposon. Tn5397 inserts at one locus, attB(Cd), in C. difficile but at multiple sites in Bacillus subtilis. Apart from a conserved 5' GA dinucleotide at the recombination site, there appears to be little sequence… CONTINUE READING