Phospholipids were extracted from the sarcoplasmic reticulum of rabbit skeletal muscle, and separated by high performance thin layer chromatography. The mole fraction of individual phospholipids were determined by assaying phosphorus of each band. Main phospholipids were scraped and extracted, and subjected to esterification by alkaline methanol, and then the composition and content of fatty acids were determined by gas chromatography. The results show that the main phospholipid components were PC (phosphatidylcholine) and PE (phosphatidylethanolamine). They account for 66.5% and 15.5%, respectively. The main fatty acid in PC is palmitic acid and those in PE are stearic acid and arachidic acid.