We have previously isolated and sequenced a portion of the structural gene for tetanus toxin from C.tetani CN3911 (1). Overlapping clones encoding the entire gene have now been isolated and the full sequence is reported below. Our designation of the start of translation is based on amino terminal analysis (2) and partial ami no acid sequence of the toxin (3). The methionine residue before the praline is presumably cleaved after synthesis. The DNA sequence contains one open reading frame encoding a protein of molecular weight 150 491, in agreement with other measurements of the size of the toxin (4).