The complete genome sequences of two isolates of potato black ringspot virus and their relationship to other isolates and nepoviruses

@article{SouzaRichards2013TheCG,
  title={The complete genome sequences of two isolates of potato black ringspot virus and their relationship to other isolates and nepoviruses},
  author={R. Souza Richards and Ian P. Adams and Jan F. Kreuze and Joao De Souza and Wilmer Jose Cuellar and Annette M. Dullemans and Ren{\'e} A.A. van der Vlugt and Rachel H Glover and Ummey Hany and M J Dickinson and Neil Boonham},
  journal={Archives of Virology},
  year={2013},
  volume={159},
  pages={811 - 815}
}
The complete nucleotide sequences of RNA 1 and RNA 2 of the nepovirus potato black ringspot virus (PBRSV) from two different isolates were determined, as well as partial sequences from two additional isolates. RNA1 is 7,579-7,598 nucleotides long and contains one single open reading frame (ORF), which is translated into a large polyprotein with 2,325 amino acids and a molecular weight of 257 kDa. The complete sequence of RNA2 ranges from 3857 to 3918 nt between the different isolates. It… 

Complete sequence and variability of a new subgroup B nepovirus infecting potato in central Peru

The data obtained support the taxonomical status of this new virus (putative named Potato virus B, PVB) as a member of the genus Nepovirus, subgroup B.

Re-examination of nepovirus polyprotein cleavage sites highlights the diverse specificities and evolutionary relationships of nepovirus 3C-like proteases

An improved understanding of the specificity of nepovirus 3C-like proteases can also be used to investigate the cleavage of plant proteins by nepov virus proteases and to understand their adaptation to a broad range of hosts.

Identification of viruses and viroids by next-generation sequencing and homology-dependent and homology-independent algorithms.

A comparison of enrichment strategies reveals that full indexing of RNA and DNA viruses as well as viroids in a plant sample at single-nucleotide resolution is made possible by one NGS run of total small RNAs, followed by data mining with homology-dependent andhomology-independent computational algorithms.

First Complete Genome Sequence of Arracacha virus A Isolated from a 38-Year-Old Sample from Peru

The first complete genomic sequence of Arracacha virus A from a Peruvian arracacha sample collected in 1975 is presented and it is shown that its RNA1 and RNA2 both had greatest amino acid identities with those of the subgroup A nepovirus Melon mild mottle virus.

Plant Virus Metagenomics: Advances in Virus Discovery.

These studies reveal a surprising lack of knowledge about plant viruses and point to the need for more comprehensive studies, which are providing useful tools to detect viruses, and perhaps to predict future problems that could threaten cultivated plants.

Viral Diseases in Potato

This chapter reviews the latest discoveries in potato virology as well as the changes in virus populations that have occurred over the last 50 years, with a particular focus on countries in the (sub-)tropics.

List of non‐EU viruses and viroids infecting potato (Solanum tuberosum) and other tuber‐forming Solanum species

The main knowledge gaps and uncertainties of this grouping concern the natural host status of potato, the taxonomy, and/or information on the geographical distribution and prevalence of some of the analysed viruses.

Current Research Topics in Plant Virology

The current status of knowledge about the viral counter-defense strategies acting at various steps during virus infection in plants with the focus on representative, well studied silencing suppressor proteins is summarized.

Diagnosis of Plant Viruses Using Next-Generation Sequencing and Metagenomic Analysis

This chapter describes the sequencing platforms and techniques used to produce and analyse NGS virus data and the barriers to this uptake including access, cost, analysis, validation and interpretation are discussed.