The compartmentation of phosphorylated thiamine derivatives in cultured neuroblastoma cells.

@article{Bettendorff1994TheCO,
  title={The compartmentation of phosphorylated thiamine derivatives in cultured neuroblastoma cells.},
  author={Lucien Bettendorff},
  journal={Biochimica et biophysica acta},
  year={1994},
  volume={1222 1},
  pages={7-14}
}
Thiamine transport in cultured neuroblastoma cells is mediated by a high-affinity carrier (KM = 40 nM). In contrast, the uptake of the more hydrophobic sulbutiamine (isobutyrylthiamine disulfide) is unsaturable and its initial transport rate is 20-times faster than for thiamine. In the cytoplasm, sulbutiamine is rapidly hydrolyzed and reduced to free thiamine, the overall process resulting in a rapid and concentrative thiamine accumulation. Incorporation of radioactivity from [14C]thiamine or… CONTINUE READING